Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging

Development of veterinary subunit vaccines comes with a spectrum challenges, such as the choice adjuvant, antigen delivery vehicle, and optimization dosing strategy. Over years, our laboratory has largely focused on investigating silica vesicles (SVs) for developing effective multiple targets. Rhipicephalus microplus (cattle tick) are known to have high impact cattle health livestock industry in tropical subtropical regions. vaccine using Bm86 against R. emerged an attractive alternative control ticks. In this study, we investigated biodistribution SV live animal model, well further explored ability development. Rhodamine-labeled SV-140-C 18 (Rho-SV-140-C ) were used adsorb Cy5-labeled (Cy5-Bm86) enable detection characterization vivo small model up 28 days optical fluorescence imaging. We tracked SVs at different timepoints (days 3, 8, 13, 28) BALB/c mice. The analysis by imaging measuring fluorescent intensity harvested organs over duration experiment (28 days) showed greater accumulation site injection. was traced lymph nodes, kidney, liver, contributing understanding how platform successfully elicits antibody responses groups administered combination SV. Selected tissues (skin, spleen, lungs) examined any cellular abnormalities histological analysis. No adverse effect or other observed tissues.